Ethnopharmacological relevance: Since the dawn of time, medicinal and aromatic plants (AMPs) represent a
precious heritage for humanity, especially in developing countries, who exploit their virtues in traditional
pharmacopoeia to cope with health problems such as diabetes, kidney stones, ulcer, and digestive disorders.
Petroselinum sativum Hoffm. belongs to Apiaceae family. It is traditionally used to treat arterial hypertension,
diabetes, cardiac disease, renal disease, and recently reported as a plant endowed with a female anti-infertility
effect.
Aim of the study: This study aims to evaluate the in vivo effect of hydro-ethanolic extract and polyphenols of
Petroselinum sativum Hoffm. on cholesterol, protein and estrogen levels, and characterize the chemical composition of polyphenolic fraction. In addition, acute toxicity and anti-inflammatory activity of tested extract was
also investigated.
Materials and methods: Chemical composition of polyphenolic fraction was determined using High-Performance
Liquid Chromatography with Diode-Array Detection (HPLC-DAD). First, toxicological investigations including
sub-acute toxicity were performed by measuring animals� weights daily for four weeks. Afterwards, histopathological examination of livers and kidneys, and serum assay of ASAT and ALAT were also checked. Next, the
acute in vivo anti-inflammatory study of the hydro-ethanolic extract and polyphenols of Petroselinum sativum
Hoffm. versus Indomethacin was conducted. Furthermore, we evaluated the estrogenic effect of its hydroethanolic extract and the polyphenolic fraction following biochemical assays for the determination of proteins, cholesterol and estrogen levels.
Results: The results revealed the presence of some phenolic compounds mainly ferulic acid, gallic acid and
quercetin. Petroselinum sativum Hoffm. extracts also showed no evidence of hepatotoxicity nor nephrotoxicity,
with remarkable anti-inflammatory activity, as well as a significant estrogenic effect compared to negative
control.
Conclusion: This study provides a scope of the potential use of Petroselinum sativum Hoffm. extracts in counteracting female infertility issues.

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